Dr. Gul Shahnaz
Group 1 A
Mechanism of Enzyme Action
Enzymes are biological catalysts that speed up the rate of the biochemical reaction.
Most enzymes are three dimensional globular proteins (tertiary and quaternary structure).
Some special RNA species also act as enzymes and are called Ribozymes e.g. hammerhead ribozyme.
STRUCTURE OF ENZYMES
The active site of an enzyme is the region that binds substrates, co-factors and prosthetic groups and contains residue that helps to hold the substrate.
Active sites generally occupy less than 5% of the total surface area of enzyme.
Active site has a specific shape due to tertiary structure of protein.
A change in the shape of protein affects the shape of active site and function of the enzyme.