This booklet deals with purification of the organic product after completion of the main reaction.
Partition Coefficient or Distribution Ratio
Thin Layer Chromatography TLC
Thin layer chromatography (TLC) is an important technique for identification and separation of mixtures of organic compounds. It is useful in:
Identification of components of a mixture (using appropriate standards)
following the course of a reaction,
analyzing fractions collected during purification,
analyzing the purity of a compound.
In TLC, components of the mixture are partitioned between an adsorbent (the stationary phase, usually silica gel, SiO2) and a solvent ( the mobile phase) which flows through the adsorbent.