Etiket Arşivleri: Thin layer chromatography

Purification Techniques ( Hermenegildo García Gómez )

This booklet deals with purification of the organic product after completion of the main reaction.


Suction Filtration

Solvent Extraction

Partition Coefficient or Distribution Ratio

Fractional Distillation

Assessing Purity


Thin Layer Chromatography TLC

Thin Layer Chromatography ( TLC )

Thin layer chromatography (TLC) is an important technique for identification and separation of mixtures of organic compounds. It is useful in:

Identification of components of a mixture (using appropriate standards)
following the course of a reaction,
analyzing fractions collected during purification,
analyzing the purity of a compound.
In TLC, components of the mixture are partitioned between an adsorbent (the stationary phase, usually silica gel, SiO2) and a solvent ( the mobile phase) which flows through the adsorbent.