‎Laboratory‎ > ‎Protein Determination

In this experiment our purpose is to determine protein concentration in a sample. For this purpose we have several methods. They are;
KJELDAHL METHOD:It can use microgram quantities of protein in a sample. They all have in comman oxidation involving material with concentrated H2SO4,ammonia is measured by distillation into standard acid. The amount of the protein is calculated by multiplying the amount of nitrogen found in the sample by a correction factor.Main disadvantage of this method is the time consuming. Also high temperature is used in this method thus this affects the structure of sample, the amount of nitrogen may be wrong because it can comes from other than the protein.H2O2, etc. In this method formed

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