1. The following data were obtained from an enzyme kinetics experiment. Graph the data using a Lineweaver-Burk plot and determine, by inspection of the graph, the values for Km and Vmax.
[S] (µM) V (nmol/min)
2. Calculate the specificity constant for an enzyme if its kcat = 1.4 x 104 s-1 Km = 90 µM. Is this enzyme very efficient?
3. Use the Michaelis-Menton Equation to calculate the missing values of [S] given below if Vmax = 5 mmol/min. Plot [S] versus V (NOT the reciprocals!). Draw line parallel to the x-axis at Vmax and extend your plotted line to show its approach to Vmax.
[S] (mM) V (mmol/min)
4. The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. Plot the data and determine, by inspection of the graph, what type of inhibition is involved.
[S] µM V (µmol/min) V (µmol/min) V (µmol/min)
with 0.0 nM with 25 nM with 50 nM
Inhibitor Inhibitor Inhibitor
______ ___________ ___________ ___________
0.4 0.22 0.21 0.20
0.67 0.29 0.26 0.24
1.00 0.32 0.30 0.28
2.00 0.40 0.36 0.32
5. You perform a kinetics experiment on the enzyme phosphatidylinositol synthase (PI synthase) using as substrate radiolabeled inositol in a tracer amount mixed with unlabeled inositol
1. Determine nmol product formed per min for each substrate concentration used.
2. Prepare a Lineweaver-Burk plot of the data.
3. Determine Km and Vmax for PI synthase.
6. (a) Define Molar (M)
(b) Define mol (or mole)
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