\r\nAlpha amylase: EC 18.104.22.168\r\nAmylases are important hydrolase enzymes which have been widely used since many decades.\u00a0These enzymes randomly cleave internal glycosidic linkages in starch molecules to hydrolyze\u00a0them and yield dextrins and oligosaccharides. Among amylases \u03b1-Amylase is in maximum\u00a0demand due to its wide range of applications in the industrial front. With consumers growing\u00a0increasingly aware of environmental issues, industries find enzymes as a good alternative over\u00a0other chemical catalysts. \u03b1-Amylase can be produced by plant or microbial sources. Due to the\u00a0advantages that microbial production offers, \u03b1-Amylase from microorganisms has been focused\u00a0upon and preferred to other sources for production. The ubiquitous nature, ease of production\u00a0and broad spectrum of applications make \u03b1-Amylase an industrially important enzyme. This\u00a0report focuses on the microbial production of \u03b1-Amylase and its applications.\r\n\uf0b7 Enzyme Production\r\nIn order to cultivate a microorganisms 4 major criteria you should be controlled or manipulate\r\n1. Temperature\r\n2. Initial pH\r\n3. Oxygen\r\n4. Carbon and Nitrogen sources\r\nIn order to control the temperature, incubator or water baths could be used. Initial pH is the\u00a0other parameter which is critical for microbial growth. As all microorganisms have optimum\u00a0temperature, they have also an optimum pH. Many microorganisms optimum pH is neutral pH\u00a0(7). The other criteria is oxygen if the microorganisms is aerobic, you should supply air to the\u00a0microorganisms. The carbon and nitrogen source is another important criteria. First of all these\u00a0C and N sources are food of microorganisms so they should able to digest and uptake both C\u00a0and N sources.\u00a0Most of the Bacillus species can synthesis and secret extracellular alpha amylase. Due to this\u00a0ability of Bacillus spesies we use Bacillus in order to obtain alpha amylase. For detection and\u00a0quantification of alpha amylase activity we use DNS method as mentioned below.