Polarimetry and Refractometry

1. Polarimeter

The technique used by polarimeter is called polarimetry, which is based on the rotation of polarized light (the light after passing a polarizer) as it passes through a sample solution (e.g. sucrose solution). Ordinary light has waves (amplitude changes cyclically) spread in all directions (planes). As light passes through a polarizer, a polarized light (a light with a narrow band width in one direction) is formed. If a solution sample changes the angle of polarized light, this indicates the presence of an optically active substance in the sample. The degree of rotation of the polarized light is called the degree of polarization. The degree of polarization is proportional to the concentration of the optically active substances.

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