Laboratory‎ > ‎Immobilization v1

PURPOSE:

The aim of this experiment is compering the reaction rate of immobilized enzyme and free enzyme to find the effectivness factor

PROCEDURE:

First of all 1 ml olive oil and 3 ml 25 mM KP buffer ( pH = 7.0 ) were putted into the flask and then 12 mg immobilized enzyme (lipase) was putted and mixed. After that ıt was placed into water bath at 37 ºC for 30 min. After 30 min the flask was centrifuged for 1 min and supernatant taken and ethanol was added on it. However, the time passed from starting centrifuge to end of adding ethanol was recorded. And then phenolphthalein was added as indicator and titrated with KOH( 0,05 N).

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