Laboratory > Gel Filtration Using Sephadex v2
When a mixture of protein applied to the top of the gel filtration column, depending on the molecular exclusion limit of column material. The large protein molecules excluded from the internal volume and thus leave the column first. The smaller protein molecules equilibrate themselves between the external and internal volume and leaves the column later.
Etiketlergel filtration column Laboratory Laboratory > Gel Filtration Using Sephadex v2 large protein molecules limit of column mixture of protein molecular exclusion smaller protein molecules