Fundamentals of Liquid Chromatography (HPLC)

First, What is Liquid Chromatography?

•  Liquid chromatography is a separation technique that involves:

•  the placement (injection) of a small volume of liquid sample

•  into a tube packed with porous particles (stationary phase)

•  where individual components of the sample are transported along  the packed tube (column) by a liquid moved by gravity.

• The components of the sample are separated from one another by the column packing that involves various chemical and/or physical interactions between their molecules and the packing particles. • The separated components are collected at the exit of this column and identified by an external measurement technique, such as a spectrophotometer that measures the intensity of the color, or by another device that can measure their amount.  F Note: The modern form of liquid chromatography is now referred to   as “flash chromatography”


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