First, What is Liquid Chromatography?
• Liquid chromatography is a separation technique that involves:
• the placement (injection) of a small volume of liquid sample
• into a tube packed with porous particles (stationary phase)
• where individual components of the sample are transported along the packed tube (column) by a liquid moved by gravity.
• The components of the sample are separated from one another by the column packing that involves various chemical and/or physical interactions between their molecules and the packing particles. • The separated components are collected at the exit of this column and identified by an external measurement technique, such as a spectrophotometer that measures the intensity of the color, or by another device that can measure their amount. F Note: The modern form of liquid chromatography is now referred to as “flash chromatography”