Enzimler

Laboratory‎ > ‎Heat Treatment v2

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In this experiment, Major affect of enzyme activity and stability is temperature. Since enzymes are biochemical catalysts, made up at least partially of protein, they are sensitive in varying degrees to heat. …

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Laboratory‎ > ‎Immobilization

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Purpose: In this experiment we determined the rate of enzyme activity using free and immobilized enzyme. And our aim in this experiment, notice the difference between them. Materials: Distilled water, …

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Laboratory‎ > ‎Km

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In this experiment we determined the Michaelis constant known as Km value that reflects the affinity of an enzyme for its substrate. A low value of Km indicates a high …

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Laboratory‎ > ‎Km v1

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Purpose: The aim of this experiment is determining Km by using three methods which are Michaelis Menten equation, Eadie Hofstee and Lineweaver Burk plot. Materials: Double beam spectrophotometer, test tubes, pipettes Enzyme …

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Laboratory‎ > ‎SDS Page

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The separation of macromolecules in an electric field is called electrophoresis. A very common method for separating proteins by electrophoresis uses a discontinuous polyacrylamide gel as a support medium and sodium dodecyl …

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Rekombinant DNA Teknolojisi

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Rekombinant-DNA teknolojisinin gelişimindeki önemli atlama taşları: 1953 Watson ve Crick : DNA’ nın çift iplikli sarmal yapısı keşfedildi. 1957 Kornberg : DNA polimeraz izole edildi. 1966 Nırenberg ve ark: Genetik …

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