Bacteriology / Genetics Lecture Notes

  • 1 DNA, GENES, AND THE CODE

  • DNA structure 4

  • Nature of genes 9

  • Homology 11

  • The genetic code 14

  • Translation 16

  • Transcription 19

  • Homologous recombination 21

  • 2 MUTAGENESIS IN VIVO

  • Inherent mutation frequency 23

  • Spontaneous mutations 24

  • Repair of all mutation types 27

  • Mutators and Anti-mutators 30

  • Chemical Mutagenesis 31

  • Systems for the analysis of mutations 33

  • Effects of mutations 35

  • Numerology 36

  • Mutant detection 37

  • 3 REGULATION

  • Themes in regulation 40

  • Regulation at the level of DNA 44

  • Regulation at the level of RNA 45

  • Regulation at the level of translation 47

  • Regulation at the level of protein 49

  • Fusions 49

  • Arrays 52

  • 4 GENETIC ENGINEERING

  • Enzymes 52

  • Vector types 53

  • PCR 56

  • Generation of mutations in vitro 56

  • In vivo analysis 57

  • 5 DELETIONS

  • Mechanism of formation 58

  • Screens/ selections for deletions 60

  • 6 DUPLICATIONS AND INVERSIONS

  • Tandem duplications 61

  • Non-tandem duplications 63

  • Amplifications 64

  • Inversions 64

  • 7 MOBILE GENETIC ELEMENTS

  • General classes of elements 66

  • Distribution and evolution of ISs 70

  • Effects of IS/Tn 71

  • Uses of Tns 72

  • 8 SELECTIONS, SCREENS AND ENRICHMENTS

  • Selections 73

  • Screens 75

  • Enrichments 76

  • 9 PLASMIDS AND CHROMOSOMES

  • Types of replicons 77

  • Replication functions 79

  • Partitioning 81

  • Conjugation 83

  • 10 TRANSFORMATION

  • Natural transformation 85

  • Induced transformation 86

  • 11 PHAGE

  • Molecular biology of phage 88

  • Uses of phage 92

  • 12 COMPLEMENTATION

  • The normal case 94

  • Interspecific complementation 98

  • 13 GENETIC MAPPING

  • Objective 99

  • Two-factor crosses 100

  • Deletion mapping 102

  • 14 SUPPRESSION

  • Introduction 103

  • Informational suppressors 104

  • Non-informational suppressors 104

  • 15 EVOLUTION 106

  • 16 SCIENCE AND SOCIETY 114

  • INDEX 118


Facebook Yorumları

Bir Cevap Yazın