• 1 DNA, GENES, AND THE CODE

• DNA structure 4

• Nature of genes 9

• Homology 11

• The genetic code 14

• Translation 16

• Transcription 19

• Homologous recombination 21

• 2 MUTAGENESIS IN VIVO

• Inherent mutation frequency 23

• Spontaneous mutations 24

• Repair of all mutation types 27

• Mutators and Anti-mutators 30

• Chemical Mutagenesis 31

• Systems for the analysis of mutations 33

• Effects of mutations 35

• Numerology 36

• Mutant detection 37

• 3 REGULATION

• Themes in regulation 40

• Regulation at the level of DNA 44

• Regulation at the level of RNA 45

• Regulation at the level of translation 47

• Regulation at the level of protein 49

• Fusions 49

• Arrays 52

• 4 GENETIC ENGINEERING

• Enzymes 52

• Vector types 53

• PCR 56

• Generation of mutations in vitro 56

• In vivo analysis 57

• 5 DELETIONS

• Mechanism of formation 58

• Screens/ selections for deletions 60

• 6 DUPLICATIONS AND INVERSIONS

• Tandem duplications 61

• Non-tandem duplications 63

• Amplifications 64

• Inversions 64

• 7 MOBILE GENETIC ELEMENTS

• General classes of elements 66

• Distribution and evolution of ISs 70

• Effects of IS/Tn 71

• Uses of Tns 72

• 8 SELECTIONS, SCREENS AND ENRICHMENTS

• Selections 73

• Screens 75

• Enrichments 76

• 9 PLASMIDS AND CHROMOSOMES

• Types of replicons 77

• Replication functions 79

• Partitioning 81

• Conjugation 83

• 10 TRANSFORMATION

• Natural transformation 85

• Induced transformation 86

• 11 PHAGE

• Molecular biology of phage 88

• Uses of phage 92

• 12 COMPLEMENTATION

• The normal case 94

• Interspecific complementation 98

• 13 GENETIC MAPPING

• Objective 99

• Two-factor crosses 100

• Deletion mapping 102

• 14 SUPPRESSION

• Introduction 103

• Informational suppressors 104

• Non-informational suppressors 104

• 15 EVOLUTION 106

• 16 SCIENCE AND SOCIETY 114

• INDEX 118

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